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Application, use, sterilization and cleaning of glass fermentation tank

Data: 2020-12-08


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Application, use, sterilization and cleaning of glass fermentation tank
Glass bioreactors are often used in scientific research institutes and microbiological laboratories of enterprises. They are suitable for the selection of microbial fermentation medium formulations, optimization of fermentation process parameters, and verification of production processes and strains. They are ideal test equipment for precision fermentation.
Borosilicate glass bioreactor
As the application range of fermentation tanks becomes wider and wider, more and more customers consult about the knowledge of glass fermentation tanks. Today, Paragon, a supplier of fermentation tanks, has summarized the application, use, sterilization and cleaning knowledge of glass fermentation tanks.

  •  Application fields of glass fermentation tank

1. Use in the food industry: There are three main categories of commodities. One is the production of traditional fermented products, such as beer, fruit wine, vinegar, etc.; the second is the production of food additives; the third is to help solve food problems.

2. Application in the pharmaceutical industry: Based on fermentation engineering skills, a variety of drugs have been developed, such as human growth hormone, recombinant hepatitis B vaccine, certain varieties of monoclonal antibodies, interleukin-2, anti-hemophilia factors, etc. .

3. Use in the field of environmental science: the enhancement of microorganisms in sewage treatment.

  •  How to use glass bioreactor

glass bioreactor from Paragon

1. Before the glass fermentation tank is used, the liquid container must be cleaned with light hot water, and then sterilized with steam. The material slurry enters the tank through the material pipe fixed on the tank cover, or opens the tank cover and pours it. 

The material should not be packed. Too full, so as not to splash the materials when being stirred, causing unsanitary or loss of the environment.

2. Heating method: When heating, the refrigerant inlet valve must be closed, put the remaining refrigerant in the jacket, and then input the material, turn on the agitator, and then turn on the steam valve.

3. After reaching the required temperature, close the steam valve first, and then close the agitator after 2-3 minutes.

4. Cooling method: close the steam valve, drain the remaining steam condensate in the jacket, and then open the refrigerant valve at the bottom of the tank. Let the refrigerant pass through the jacket to reduce the temperature of the material in the tank.

5. Insulation: According to the required temperature, start the stirrer, adjust the valve, and maintain the temperature, (pay attention to the thermometer), in order to achieve the purpose of insulation.

  •  Sterilization method of glass fermentation tank

"Sterilization" refers to the technology or process that uses chemical or physical methods to kill or remove all living substances in materials and equipment. Sterilization can essentially be divided into two types: sterilization and lysis. The former refers to the death of bacteria. But the body still exists, the latter refers to the phenomenon that the cells melt and disappear after the bacteria are killed.

Commonly used sterilization techniques are generally divided into: physical sterilization and chemical sterilization. Physical sterilization also includes moist heat sterilization, dry heat sterilization, radiation sterilization and filter sterilization. 

The chemical sterilization is mainly to use chemical reagents (such as formaldehyde, phenol, triclosan, peracetic acid, potassium permanganate, etc.) to sterilize certain containers or materials and sterile areas.

In terms of biological fermentation, the commonly used equipment is mechanical agitation fermentation, and its sterilization effect directly affects whether the fermentation can proceed normally.

  • The cleaning method of the glass fermentation tank

The glass fermentation tank should be carefully cleaned before and after use, especially when different strains are used for the two cultures before and after, more attention should be paid to cleaning and sterilization. 

Any part that can be cleaned in the glass fermentation tank should be carefully cleaned, otherwise it may become a breeding ground for bacteria. 

The places that are easily overlooked and cannot be cleaned sufficiently include the inside of the nozzle corner, the sampling tube, and the top of the tank.