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Fed-Batch Cultivation

Data: 2020-08-01

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Fed-Batch Cultivation

In fed batch cultivation, one or more nutrients are added aseptically, it is a semi-open system and the culture is supplemented step-by-step into the bioreactor at the same time the volume of the liquid culture in the bioreactor increase within this time.


The increase in productivity, enhanced yield by controlled sequential addition of nutrients, ability to achieve higher cell densities, and pro-longed product synthesis are the main advantages of fed-batch over batch cultures.

Immobilized Cell Technology Active Biocatalyst also known as enzyme or microbial cell has increased the productivity of bioprocesses and it is managed through controlled contact with high concentration.


Through cell immobilization or recycling by feeding strategies in high density cultures. Cell immobilization mostly studied in the food and gas-liquid mass. It is done in three phase bioreactor; it requires all three phases in competent mass transfer. These bioreactor aims in the region where main process amplification can be managed through the improvement of gas-liquid mass transfer.


Fundamental difference between SmF and SSF


Submerged fermentation

Solid-state (substrate) fermentation

Water cultivation medium (~95%).

Water cultivation medium is low

(40–80%).

Liquid–gas are the two phase of the

system.

Solid–liquid–gas are three phase of

system.

Homogeneous.

Homogeneous.

Low nutrient content, water soluble.

High nutrient content, water

insoluble.

Oxygen transfer: gas–liquid.

Oxygen transfer: liquid–solid and

gas–liquid.

Microorganism growth: liquid

medium.

Microorganism growth: medium

surface.

Only oxygen is transfer, process is not

limited.

Oxygen, heat, and nutrient transfer is

limited.

Product: soluble in the liquid phase.

Product: high concentration.

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