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Microbial Fermenter&Bioreactor

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Pilot-Scale Microbial fermenter

This product is widely applied in pilot scale production in enterprises, It can be used to select the medium formula, optimize the fermentation process parameters and validation of the production process. Various volumes fermenter are freely combined, and special specifications can be customized.
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Description

This product is widely applied in pilot scale production in enterprises, It can be used to select the medium formula, optimize the fermentation process parameters and validation of the production process. Various volumes fermenter are freely combined, and special specifications can be customized.

Parameter

Item

Specification

Nominal

Capacity

15L; 20L; 30L; 50L; 100L;

Liquid

Culture

Loading

ratio

65%-80%, 70% generally;

Vessel

1.Design Pressure: 0.25Mpa, Working Pressure0.15Mpa;

2.Maximum Design Temperature: 135, Working Temperature: 121;

3. ll segment touch with culture solution: S31603; Other parts: S30408

4. Jacket for temperature control;

5. Internal surface roughnessRa0.6μm; no surface deficiency, no blind angle;

External surface: polishing and matte processing (or glass shot peening);

6. Viewing port: illuminated by 12V LED safety light;

7. Reserved ports for pH electrode, DO electrode and temperature electrode;

8. Reserved ports in head cover for inoculation and culture feeding;

Agitation

System

1. Upper agitation design, S31603 agitating frame;

2. Aseptic mechanical sealing;

3. Equipped with 2 layers flat-straight blade impeller, 1 layer defoaming paddle; 

(Ps: inclined blade, curved blade, and axial flow blade for option)

4.  Stepless speed regulation, digital setting speed control;

Structural

Style

Desk-mounted / ground-mounted

Aeration

System

1. Air inlet from the bottom of vessel, and equipped with circular air distributor;

2. Displayed by glass style flowmeter and pressure gage, regulating air flow by manual valve;

3. Sterilizing filter. Brand: PALL (USA), accuracy: 0.2 μm;

4. Air compressor with dehydration pressurizer for supplying the air stably and constantly;

Optional: mass flow controller for controlling the air flow automatically.

Temperature

Control

1. Online detection, digital setting, auto/manual control;

2. Pt-100 temperature probe, displayed in digital;

3. Control the temperature automatically by cyclic heating water and cooling water.

pH

1. Online detection by pH electrode which can be sterilized by vapor;

2. Feeding alkali and acid to regulate pH through #1 or #2 peristaltic pump.

DO

1. Online detection by DO electrode which can be sterilized by vapor;

2. Control range: 0-100% or 0-200%;

Optional: linkage control with agitating speed.

Culture

Feeding

Standard equipped one channel of peristaltic pump for fed-batch culture by time proportioning, fed-batch accumulated amount recorded and displayed.

Defoaming

1.  Defoaming electrode, automatic alarming when foam abnormal;

2.  Standard equipped one channel of peristaltic pump for fed-batch defoamer automatically or manually, 

fed-batch accumulated amount recorded and displayed.

Control

System

Adopt industrial grade WFBio control system, Siemens PLC  Smart200 controller, 32 bit true color touch screen interface, full function, stable operation (C model); plus A model sub-instrument control system is optional.

Sterilization

1.  Sterilization in place, manual control. optional: automatic control;

2.  We provide vapor generator for sterilization;

Optional

1.  Multi-channels feeding;

2.  Automatic sterilization (auto-SIP);

3.  Head cover auto-lift system (head cover can be lifted 500mm height by electrical or pneumatic);

4.  Culture feeding weighing;

5.  Vessel weighing;

6.  Bypass for oxygen inlet;

7.  Online detection of redox potential;

8.  Air flow detected and controlled automatically;

9.  Online detection of O2 and CO2 in tail gas;

10. Online detection of methanol and ethanol;

11. Online detection of glucose and amino acid.

Feature

Sterilization in place, safety and reliable;

Accurate detection and control of culture concentration;

clear observation;

Various ways of inoculation (flame inoculation and differential pressure inoculation);


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