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Microbial Fermenter&Bioreactor

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Plant-Scale Microbial fermenter

Plant-Scale fermenter is mainly for the commercial production scale in many fields, e.g. fertilizer; biological production; food; alcohol; pharmaceutical production; etc. special specifications can be customized.
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Description


Plant-Scale fermenter is mainly for the commercial production scale in many fields, e.g. fertilizer; biological production; food; alcohol; pharmaceutical production; etc. special specifications can be customized.


Parameter


Item


Specification


Nominal


Capacity


 200L; 500L; 1000L; 2000L; 5000L; 10m³;20m³; 50m³; etc.

Liquid  Culture Loading  ratio

65%-80%, 70% generally;

Vessel


Design Pressure: 0.25Mpa, Working Pressure0.15Mpa;
Maximum Design Temperature: 135, Working Temperature: 121;
All segment touch with culture solution: S31603; Other parts: S30408
Jacket for temperature control;
Internal surface roughnessRa0.6μm; no surface deficiency, no blind angle;
External surface: polishing and matte processing (or glass shot peening);

Viewing port: illuminated by 12V LED safety light;
Reserved ports for pH electrode, DO electrode and temperature electrode;
Reserved ports in head cover for inoculation and culture feeding;
Agitation


System


Upper agitating design, S31603 agitating frame;
Aseptic mechanical sealing;
Equipped with 2 layers flat-straight blade impeller, 1 layer defoaming paddle; (Ps: inclined blade, curved blade, and axial flow blade for option)
Stepless speed regulation, digital setting speed control;
Structural


Style


 Desk-mounted / ground-mounted

Aeration


System


Air inlet from the bottom of vessel, and equipped with circular air distributor;
Displayed by glass style flowmeter and pressure gage, regulating air flow by manual valve;
Sterilizing filter. Brand: PALL (USA), accuracy: 0.2 μm;
Air compressor with dehydration pressurizer for supplying the air stably and constantly;

      Optional: mass flow controller for controlling the air flow automatically.

Temperature


Control


Online detection, digital setting, auto/manual control;
Pt-100 temperature probe, displayed in digital;
Control the temperature automatically by cyclic heating water and cooling water.
pH


Online detection by pH electrode which can be sterilized by vapor;
Feeding alkali and acid to regulate pH through #1 or #2 peristaltic pump.
DO


Online detection by DO electrode which can be sterilized by vapor;
Control range: 0-100% or 0-200%; Optional: linkage control with agitating speed.
Culture


Feeding


Standard equipped one channel of peristaltic pump for fed-batch culture by time proportioning, fed-batch accumulated amount recorded and displayed.

Defoaming


Defoaming electrode, automatic alarming when foam abnormal;
Standard equipped one channel of peristaltic pump for fed-batch defoamer automatically or manually, fed-batch accumulated amount recorded and displayed.
Control


System


Adopt industrial grade WFBio control system, Siemens PLC  Smart200 controller, 32 bit true color touch screen interface, full function, stable operation (C model); plus A model sub-instrument control system is optional.

Sterilization


Sterilization in place, manual control. optional: automatic control;
We provide vapor generator for sterilization;

Optional


Multi-channels feeding;
Automatic sterilization (auto-SIP);
Head cover auto-lift system (head cover can be lifted 500mm height by electrical or pneumatic);
Culture feeding weighing;
Bypass for oxygen inlet;
Online detection of redox potential;
Air flow detected and controlled automatically;
Online detection of O2 and CO2 in tail gas;
Online detection of methanol and ethanol;
Online detection of glucose and amino acid.
Feature

Sterilization in place, safety and reliable;

Accurate detection and control of culture concentration;

clear observation;

 Various ways of inoculation (flame inoculation and differential pressure inoculation);