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Type of microbial reactor

Data: 2020-11-26


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Type of microbial reactor

Mechanical agitation bioreactor: 

The principle is to use mechanical agitation to suspend and ventilate cells; the structure of the reactor is generally composed of a cylindrical outer wall and a vertical additional impeller on the central axis. Its main advantages are: sufficient stirring, feeding and mixing effects Well, the dissolved oxygen coefficient KLa>100/h, the temperature, pH and nutrient concentration in the reflector are easier to adjust than other reactors, and the experience of microbial culture can be directly used for research and control.

The biological enzyme reactor is mainly suitable for cells with strong shear tolerance, such as tobacco cells, jellyfish snow lotus cells, etc.;

Since the cell wall of most plant cells is sensitive to shearing force and is easy to cause cell damage, it is necessary to improve the stirring blade when using a stirred reactor. Generally, it can be reduced by changing the stirring form, impeller structure and type, etc. Shear force due to stirring.

Non-mechanically stirred reactor:

(1) Airlift reactor/fermentor

Airlift reactor is a type of bioreactor that uses the updraft of sterile air from the reactor to drive the culture liquid to circulate, so that the two functions of feeding and mixing are integrated. 

According to the different structure, it can be divided into two forms: outer circulation and inner circulation.

The bio-enzyme reactor can be divided into two major areas: ascending liquid zone and descending liquid zone, mainly including the deflector tube, the gas-liquid separation device at the top of the tower, and the gas distribution device at the bottom of the tower, and several parts of the gas are introduced by the gas distribution device at the bottom of the tower. 

The deflector tube is discharged at the top of the tower after gas-liquid separation, so as to drive the liquid to circulate in the tower from the rising liquid zone to the falling liquid zone to achieve the purpose of stirring.

Since the biological enzyme reactor has no stirring device, the shearing force is small, the damage to plant cells is small, the culture is also continuously circulated, and the mixing effect is good, which is beneficial to increase the cell concentration and the production of secondary metabolites. It is the most suitable for plants. One of the bioreactors for cell culture.

(2) Bubbling reactor: 

A type of reactor that uses the sterile air introduced at the bottom of the reactor to produce a large amount of foaming, and plays the role of feeding and mixing during the ascent process; no mechanical energy consumption is required during the cultivation process , Suitable for culturing cells sensitive to shear force,

(3) Rotary drum reactor: 

It is a new type of bioreactor that achieves the purpose of mixing through the rotation of the turntable or the rotating stock; it is characterized by a uniform suspension system, a low shear environment, high feeding efficiency, and prevention.

The advantage of cell adhesion on the wall is suitable for high-density plant suspension culture.

Plant cell scale microbial enzyme reactor:

(1) Fluidized bed bioreactor: 

Uses the energy of the fluid to make the support particles in a suspended fluidized state, and the immobilized cells and bubbles are suspended in the culture solution to absorb nutrients and cultivated. 

Its characteristics are: simple structure and high transfer coefficient. The reactants are evenly mixed

(2) Packed bed bioreactor: 

Fix the cells on the surface or inside of the support, stack the support particles into a bed, immobilize, and the medium flows between the beds to realize the transfer and mixing of substances. 

Its characteristics are: unit volume The cell density is large, which can meet the characteristics of plant cell population growth. 

The disadvantage is that the mixing effect is poor, the mass transfer efficiency is low, and the particle support in the bottom of the bed is easily broken or deformed under high pressure to block the bed.